This study evaluated the production of hydrogen in anaerobic fluidized bed reactor (RALF) treating a synthetic wastewater containing glucose. The reactor was built in acrylic with internal diameter of 5.3 cm and height of 190 cm. The inoculum was used sludge from UASB reactor treating waste of swine production. The reactor has a total volume of 4192 cm3, and 2490 cm3 was occupied by support from detention of biomass. Particles of expansive clay (2.5 mm) were used as a means of support detention of biomass. The reactor was operated with synthetic substrate, containing glucose as the sole carbon source to a concentration of 2000 mg/L. The RALF was operated with hydraulic retention time (HRT), 8, 6, 4, 2 and 1 h at a temperature of 30 °C. The performance of the unit was assessed on parameters for the production of hydrogen, concentration of glucose, chemical oxygen demand (COD), alkalinity, total volatile acids, pH. It was the maximum rate of production of hydrogen from 0.97 L/h/L and a maximum yield (YH2/glicose) from 3.43 mol H2/mol glucose. The biogas produced was composed of H2 and CO2. Composition of H2 increased from 8 % to 27 % with a reduction of HRT, reaching maximum value of 35 %. Overall, the results show the potential of the proposed system to produce hydrogen.