Ti₂₅V₃₅Cr₄₀ alloy exhibits a superior maximum hydrogenation capacity of 1.56 H/M, but its effective reversible hydrogen content for applications at 30 ^oC is only approximately 0.73 H/M. To increase its effective hydrogen storage content, an approach to promote hydrogen desorption pressure of hydride by addition of interstitial elements such as boron and carbon was attempted, which has been recognized effectively in raising hydrogen desorption pressure for TiFe-based alloys previously. In this study, Ti₂₅V₃₅Cr₄₀, Ti₂₅V₃₅Cr₄₀B₅ and Ti₂₅V₃₅Cr₄₀C₅ alloys were prepared by arc-melting and annealed in vacuum at 1200 ^oC for 2 h to eliminate segregation. X-ray diffraction results indicate that all of specimens were crystalline with a bcc structure but came with a minor second phase due to the doping of B and C. For addition of B with r_a = 0.046 nm to Ti₂₅V₃₅Cr₄₀, the desorption pressure of second plateau in pressure-composition isotherm curve at 30 ^oC was elevated slightly from 177 to 269 kPa, thereby enhancing the effective reversible hydrogen content to 0.80 H/M. On the other hand, for addition of C with r_a = 0.077 nm, the desorption pressure of second plateau was increased significantly from 177 to 1275 kPa. The corresponding effective reversible hydrogen content was lowered to 0.64 H/M because of losing available interstitial sites for H storage in the lattice. Therefore, the effective hydrogen content of Ti₂₅V₃₅Cr₄₀ may be influenced with the doped interstitial atomic sizes of B and C elements.